Journal
/
Bioengineering
/
Deteksjon av Endotoxin i Nano-formuleringer bruker Limulus Amoebocyte Lysate (LAL) analyser
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays

Deteksjon av Endotoxin i Nano-formuleringer bruker Limulus Amoebocyte Lysate (LAL) analyser

DOI:
10.3791/58830-v

•

06:15 min

•

January 30, 2019

•

,
1Nanotechnology Characterization Lab., Cancer Research Technology Program,Frederick National Laboratory for Cancer Research sponsored by National Cancer Institute

Chapters

  • 00:04Title
  • 00:32Turbidity Limulus Amoebocyte Lysate (LAL) Assay
  • 02:26Sample Loading and Preparation and Gel-Clot LAL
  • 04:27Results: Representative Detection of Endotoxin in PEGylated Liposomal Doxorubicin Using LAL Assays
  • 05:12Conclusion

Summary

Automatic Translation

Automatic Translation

Deteksjon av endotoxins i utvikling nanomaterialer representerer en av de store utfordringene innen nanomedicine. Her presenterer vi en studie som beskriver rammeverket består av tre ulike LAL formater å beregne potensiell endotoxin forurensning i nanopartikler.

Tags

EndotoxinNano-formulationsLimulus Amoebocyte Lysate (LAL) AssaysCalibration StandardControl Standard EndotoxinLAL Grade WaterQuality ControlInstrument SettingsTest IDData GroupHardwareLAL MethodSerial NumberSystem IDSerial PortSample IDNegative ControlStandard CurveTest SamplesInhibition Enhancement ControlDilution

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Developing Custom Chinese Hamster Ovary-host Cell Protein Assays using Acoustic Membrane Microparticle Technology

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Deteksjon og Isolering av Sirkulerende Melanom Cells bruke photoacoustic Flowmetry

Bakteriell Detection & Identification hjelp elektrokjemiske sensorer

Flexural Rigidity Measurements of Biopolymers Using Gliding Assays

Kvantifisering av Global diastolisk funksjon av Kinetisk modellering basert analyse av transmitral Flow via Parametrized diastolisk Fylling formalisme

Perle aggregering Analyser for karakterisering av Antatte celleadhesjonsmolekyler

Øyeirritasjonstest (EIT) for fareidentifikasjon for Eye irriterende kjemikalier som bruker Rekonstruert Menneskelig Hornhinnen lignende Epithelial (RHCE) Tissue Modell

Etikett-free enkelt molekyl Detection Bruke Microtoroid Optiske resonatorer

Multicolor Fluorescence Detection for Droplet Microfluidics Bruke optiske fibre

Read Article