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Lipid tolagede eksperimenter med kontakt boble dobbeltlag for Patch-Clampers
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Lipid tolagede eksperimenter med kontakt boble dobbeltlag for Patch-Clampers

Article DOI: 10.3791/58840-v • 07:18 min • January 16th, 2019
January 16th, 2019 •
1, 1
1Department of Molecular Physiology and Biophysics, University of Fukui Faculty of Medical Sciences

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Vi præsenterer her, en protokol for dannelsen af lipid dobbeltlag ved hjælp af en kontakt boble tolagede metode. En vand boble er blæst ind i et organisk opløsningsmiddel, hvorved en éncellelag dannes på grænsefladen vand-olie. To pipetter er manipuleret til at forankre boblerne til at danne en tolagede.

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Neurovidenskab spørgsmålet 143 Lipid tolagede Patch Clamp vand-i-olie dråbe éncellelag Ion-kanal Elektrofysiologi overfladekemi
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