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Journal / Cancer Research / Rilevazione in tempo reale nell'apoptosi delle…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
Rilevazione in tempo reale nell'apoptosi delle cellule tumorali in Vitro indotta da CD8+ T cellule per studiare funzioni immuni soppressive di tumore-infiltrazione di cellule mieloidi
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Rilevazione in tempo reale nell'apoptosi delle cellule tumorali in Vitro indotta da CD8+ T cellule per studiare funzioni immuni soppressive di tumore-infiltrazione di cellule mieloidi

Article DOI: 10.3791/58841-v • 09:57 min • January 29th, 2019
January 29th, 2019 •
*1,2, *3, 2, 3,4
1Royal (Dick) School of Veterinary Studies and Roslin Institute, University of Edinburgh, 2MRC Centre for Reproductive Health, University of Edinburgh, 3Edinburgh Phenotypic Assay Centre, University of Edinburgh, 4Cancer Research UK Edinburgh Centre, MRC Institute of Genetics, Molecular Medicine, University of Edinburgh
* These authors contributed equally

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Descriviamo qui un protocollo per studiare la citotossicità di pre-attivato CD8+ T cellule contro le cellule tumorali dalla rilevazione apoptotic microscopia del cancro cellule via in tempo reale. Questo protocollo può studiare i meccanismi dietro la soppressione delle cellule T indotta da cellula mieloide e valutare composti puntati replenishing T cellule tramite blocco delle cellule mieloidi soppressive immuni.

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Ricerca sul cancro numero 143 apoptosi cellula tumorale CD8+ T cellulare citotossicità microscopia a fluorescenza cellule mieloidi-derivato del soppressore macrofagi tumore-collegati
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