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Journal / Cancer Research / Detecção de tempo real em Vitro Tumor celular…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
Detecção de tempo real em Vitro Tumor celular apoptose induzida por CD8+ T células para estudar as funções imunológicas supressora de Tumor infiltrando células mieloides
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Detecção de tempo real em Vitro Tumor celular apoptose induzida por CD8+ T células para estudar as funções imunológicas supressora de Tumor infiltrando células mieloides

Article DOI: 10.3791/58841-v • 09:57 min • January 29th, 2019
January 29th, 2019 •
*1,2, *3, 2, 3,4
1Royal (Dick) School of Veterinary Studies and Roslin Institute, University of Edinburgh, 2MRC Centre for Reproductive Health, University of Edinburgh, 3Edinburgh Phenotypic Assay Centre, University of Edinburgh, 4Cancer Research UK Edinburgh Centre, MRC Institute of Genetics, Molecular Medicine, University of Edinburgh
* These authors contributed equally

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Descrevemos aqui um protocolo para investigar a citotoxidade de CD8 pré-activated+ T células contra as células cancerosas através da detecção de apoptotic câncer células através do tempo real microscopia. Este protocolo pode investigar os mecanismos subjacentes mieloide induzida por células T cell supressão e avaliar compostos vistos reabastecer T células através do bloqueio de células mieloides supressivas do sistema imunológico.

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Cancer Research edição 143 apoptose a célula cancerosa CD8+ T célula citotoxicidade microscopia de fluorescência células supressoras mieloide-derivado macrófagos tumor-associados
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