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Journal / Immunology and Infection / Flerfarget flyt cytometri-baserte kvantifisering…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Flerfarget flyt cytometri-baserte kvantifisering av mitokondrier og lysosomer i T-celler
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Flerfarget flyt cytometri-baserte kvantifisering av mitokondrier og lysosomer i T-celler

Article DOI: 10.3791/58844-v • 06:22 min • January 9th, 2019
January 9th, 2019 •
1, 1, 1, 1
1Institute of Microbiology and Immunology, National Yang-Ming University

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Denne artikkelen beskriver en kraftig metode for å kvantifisere mitokondrier eller lysosomer i levende celler. Kombinasjonen av lysosome - eller mitokondrier-spesifikk fargestoffer med fluorescently konjugert antistoffer mot overflate markører lar kvantifisering av disse organeller mikset-celle bestander, som primær celler høstet fra vevsprøver, ved hjelp av flerfarget flowcytometri.

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Immunologi og infeksjon problemet 143 flyt cytometri multicolor T-celler lysosome mitokondrier organeller-spesifikke fargestoffer
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