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JoVE Journal Neuroscience
Forberedelse af rytmisk-aktiv i Vitro Neonatal gnaver hjernestammen-rygmarven og tynd skive
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Forberedelse af rytmisk-aktiv i Vitro Neonatal gnaver hjernestammen-rygmarven og tynd skive

Article DOI: 10.3791/58870-v • 06:32 min • March 23rd, 2019
March 23rd, 2019 •
1,2, 1, 3, 1
1Center for Perinatal Biology, Department of Basic Sciences, Loma Linda University, 2Department of Biology, The College of New Jersey, 3Department of Neurosurgery, Loma Linda University

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Denne protokol både visuelt kommunikerer hjernestammen rygmarven forberedelse og præciserer forberedelse af hjernestammen tværgående skiver på en omfattende måde, trin for trin. Det var designet til at øge reproducerbarhed og øge sandsynligheden for at få levedygtigt, langtidsholdbare, rytmisk-aktive skiver til optagelse af neurale output fra regionerne respiratorisk af hjernestammen.

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Neurovidenskab sag 145 hjernestammen-spinal ledning dissektion vibratome skæring en blok optagelse in vitro- skive kraniel finrødder cervikal finrødder rytmisk-aktive skive respiratorisk mønster generation
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