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Neuroscience

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JoVE Journal Neuroscience
Sondera Nicotinic acetylkolin Receptor funktion i mus hjärnan skivor via Laser Flash fotolys Photoactivatable nikotin
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Sondera Nicotinic acetylkolin Receptor funktion i mus hjärnan skivor via Laser Flash fotolys Photoactivatable nikotin

Article DOI: 10.3791/58873-v • 10:48 min • January 25th, 2019
January 25th, 2019 •
1, 2, 3, 3, 1
1Department of Pharmacology, Northwestern University Feinberg School of Medicine, 2Department of Physiology, Northwestern University Feinberg School of Medicine, 3Janelia Research Campus, Howard Hughes Medical Institute

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Denna artikel presenterar en metod för att studera nikotinacetylkolinreceptorer (nAChRs) i mus hjärnan skivor av nikotin uncaging. När tillsammans med samtidiga patch clamp inspelning och 2-foton mikroskopi för laserscanning, ansluter nikotin uncaging nikotinhaltiga receptorn funktion med cellulära morfologi, att ge en djupare förståelse av kolinerga neurobiologi.

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Neurovetenskap fråga 143 fotolys uncaging elektrofysiologi receptor imaging nikotin kolinerga nikotinhaltiga 2-photon acetylkolin
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