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JoVE Journal Chemistry
Escherichia coli-base della sintesi proteica Cell-Free
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Escherichia coli-base della sintesi proteica Cell-Free: protocolli per la tecnologia della piattaforma robusta, flessibile e accessibile

Article DOI: 10.3791/58882-v • 09:45 min • February 25th, 2019
February 25th, 2019 •
*1,2, *2,3, 4,5,6,7, 2,3, 2,3
1Department of Biological Sciences, California Polytechnic State University, San Luis Obispo, 2Center for Applications in Biotechnology, California Polytechnic State University, San Luis Obispo, 3Department of Chemistry and Biochemistry, California Polytechnic State University, 4Department of Chemical and Biological Engineering, Northwestern University, 5Chemistry of Life Processes Institute, Northwestern University, 6Center for Synthetic Biology, Northwestern University, 7Interdisciplinary Biological Sciences Program, Northwestern University
* These authors contributed equally

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Questo protocollo dettaglia i passaggi, i costi e attrezzature necessarie per generare e. coli-base di estratti cellulari e implementare in vitro reazioni di sintesi di proteine entro 4 giorni o meno. Per sfruttare la natura flessibile di questa piattaforma per vaste applicazioni, discutiamo le condizioni di reazione che possono essere adattate e ottimizzate.

Tags

Chimica problema 144 Cell-free proteina sintesi CFPS in vitro l'espressione della proteina High-throughput proteina sintesi TX-TL biologia sintetica Cell-free metaboliche ingegneria CFME
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