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Journal / Chemistry / Escherichia coli-base de la synthèse protéique…
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Escherichia coli-base de la synthèse protéique acellulaire 
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Escherichia coli-base de la synthèse protéique acellulaire : protocoles pour une technologie de plate-forme robuste, flexible et accessible

Article DOI: 10.3791/58882-v • 09:45 min • February 25th, 2019
February 25th, 2019 •
*1,2, *2,3, 4,5,6,7, 2,3, 2,3
1Department of Biological Sciences, California Polytechnic State University, San Luis Obispo, 2Center for Applications in Biotechnology, California Polytechnic State University, San Luis Obispo, 3Department of Chemistry and Biochemistry, California Polytechnic State University, 4Department of Chemical and Biological Engineering, Northwestern University, 5Chemistry of Life Processes Institute, Northwestern University, 6Center for Synthetic Biology, Northwestern University, 7Interdisciplinary Biological Sciences Program, Northwestern University
* These authors contributed equally

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Ce protocole détaille les étapes, les coûts et équipements nécessaires pour générer des e. coli-extraits cellulaires de base et mettre en œuvre en vitro réactions de synthèse de protéines dans les 4 jours ou moins. Pour tirer parti de la souplesse de cette plate-forme pour les applications larges, nous discutons des conditions de réaction qui peuvent être adaptées et optimisées.

Tags

Chimie numéro 144 acellulaire synthèse CFPS in vitro protein expression protéique High-throughput protein synthèse TX-TL biologie synthétique acellulaire métabolique ingénierie FCMO
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