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Journal / Biochemistry / En Fluorogenic peptid klyvning analysen till…
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Biochemistry

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JoVE Journal Biochemistry
En Fluorogenic peptid klyvning analysen till skärmen för den proteolytiska aktiviteten
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En Fluorogenic peptid klyvning analysen till skärmen för den proteolytiska aktiviteten: program för coronavirus spike protein aktivering

Article DOI: 10.3791/58892-v • 07:53 min • January 9th, 2019
January 9th, 2019 •
*1,2, *1,3, 1,4, 1, 1
1Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, 2Department of Microbiology, College of Agricultural and Life Sciences, Cornell University, 3Virologie et Immunologie Moléculaires, Domaine de Vilvert, INRA, 4Department of Cell Biology and Molecular Genetics, University of Maryland
* These authors contributed equally

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Vi presenterar en fluorogenic peptid klyvning analysmetod som tillåter en snabb screening av den proteolytiska aktiviteten av proteaser på peptider som representerar webbplatsen klyvning av viral fusion peptider. Denna metod kan också användas på någon annan aminosyra motiv inom en proteinsekvens för att testa för aktiviteten proteashämmare.

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Biokemi fråga 143 peptid analys Coronavirus felint coronavirus MERS-CoV Fusion peptid proteas klyvning Furin
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