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Journal / Biochemistry / 一种用于蛋白质溶解活性的荧光肽裂解检测:…
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Biochemistry

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JoVE Journal Biochemistry
一种用于蛋白质溶解活性的荧光肽裂解检测
High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
Journal (Biochemistry)
High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA)
Journal (Biochemistry)
Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA)
 
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一种用于蛋白质溶解活性的荧光肽裂解检测: 冠状病毒穗蛋白活化的应用

Article DOI: 10.3791/58892-v • 07:53 min • January 9th, 2019
January 9th, 2019 •
*1,2, *1,3, 1,4, 1, 1
1Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, 2Department of Microbiology, College of Agricultural and Life Sciences, Cornell University, 3Virologie et Immunologie Moléculaires, Domaine de Vilvert, INRA, 4Department of Cell Biology and Molecular Genetics, University of Maryland
* These authors contributed equally

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Summary
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我们提出了一个氟原肽裂解检测, 允许快速筛选蛋白酶的蛋白溶解活性肽代表病毒融合肽的裂解部位。这种方法也可以用于蛋白质序列中的任何其他氨基酸母题, 以测试蛋白酶活性。

Tags

生物化学 第143期 多肽检测 冠状病毒 猫冠状病毒 中东呼吸综合征冠状病毒 融合肽 蛋白酶裂解 呋喃
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