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Isolering, rensning og differentiering af Osteoklastprækursorer fra rotte knoglemarv
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Isolering, rensning og differentiering af Osteoklastprækursorer fra rotte knoglemarv

Article DOI: 10.3791/58895-v • 11:11 min • May 19th, 2019
May 19th, 2019 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 3, 3, 1,2,4
1Laboratory of New Techniques of Restoration & Reconstruction, Institute of Traumatology & Orthopedics, Nanjing University of Chinese Medicine, 2TCM Nursing Intervention Laboratory of Chronic Disease Key Laboratory, Nanjing University of Chinese Medicine, 3Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, 4Department of Traumatology and Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine

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Osteoklaster er vævsspecifikke makrofag polykaryoner afledt af monocyte-makrofag-Lineage af hæmatopoietiske stamceller. Denne protokol beskriver, hvordan man isolerer knoglemarvsceller, så der opnås store mængder osteoklaster, samtidig med at risikoen for ulykker, der findes i traditionelle metoder, mindskes.

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Biologi osteoklast osteoporose receptor aktivator for nuklear faktor-κB ligand (RANKL) knogle Remodeling cellebiologi isolation knogleresorption
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