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JoVE Journal Bioengineering
Lucifer Yellow-en robust paracellular permeabilitet markør i en celle modell av Human Blood-Brain Barrier
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Lucifer Yellow-en robust paracellular permeabilitet markør i en celle modell av Human Blood-Brain Barrier

Article DOI: 10.3791/58900-v • 06:22 min • August 19th, 2019
August 19th, 2019 •
1, 1, 2, 1
1Department of Pharmaceutical, Administrative and Social Sciences, Duquesne University, 2Department of Pharmaceutical Sciences, University of Kentucky

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Vi presenterer en fluorescens analysen for å demonstrere at Lucifer Yellow (LY) er en robust markør for å fastslå den tilsynelatende paracellular permeabilitet av hCMEC/D3 celle monolagere, en in vitro modell av den menneskelige blod-hjerne-barriere. Vi brukte denne analysen for å bestemme Kinetics av en confluent monolag formasjon i kultivert hCMEC/D3 celler.

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Bioteknologi Lucifer gul paracellular permeabilitet blod-hjerne barriere hCMEC/D3 DNA nanopartikler transfeksjoner.
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