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JoVE Journal Bioengineering
Lucifer Yellow-en robust Paracellulær permeabilitet markør i en celle model af humant blod-hjerne barrieren
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Lucifer Yellow-en robust Paracellulær permeabilitet markør i en celle model af humant blod-hjerne barrieren

Article DOI: 10.3791/58900-v • 06:22 min • August 19th, 2019
August 19th, 2019 •
1, 1, 2, 1
1Department of Pharmaceutical, Administrative and Social Sciences, Duquesne University, 2Department of Pharmaceutical Sciences, University of Kentucky

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Vi præsenterer en fluorescens analyse for at påvise, at Lucifer Yellow (LY) er en robust markør til at bestemme den tilsyneladende paracellulære permeabilitet af hCMEC/D3 Cell monolayers, en in vitro model af den menneskelige blod-hjerne barriere. Vi brugte denne analyse til at bestemme kinetikken af en konfluent enkeltlags dannelse i kulturperler hcmec/D3 celler.

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Bioteknik Lucifer gul paracellulær permeabilitet blod-hjerne barrieren hCMEC/D3 DNA nanopartikler transfection.
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