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JoVE Journal
Bioengineering

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Lucifer Yellow-en robust Paracellulær permeabilitet markør i en celle model af humant blod-hjerne barrieren
 

Lucifer Yellow-en robust Paracellulær permeabilitet markør i en celle model af humant blod-hjerne barrieren

Article doi: 10.3791/58900
August 19th, 2019

Summary August 19th, 2019

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Vi præsenterer en fluorescens analyse for at påvise, at Lucifer Yellow (LY) er en robust markør til at bestemme den tilsyneladende paracellulære permeabilitet af hCMEC/D3 Cell monolayers, en in vitro model af den menneskelige blod-hjerne barriere. Vi brugte denne analyse til at bestemme kinetikken af en konfluent enkeltlags dannelse i kulturperler hcmec/D3 celler.

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