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Journal / Immunology and Infection / Induktion av mus lung skada genom endotrakeal…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Induktion av mus lung skada genom endotrakeal injektion av bleomycin
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Induktion av mus lung skada genom endotrakeal injektion av bleomycin

Article DOI: 10.3791/58922-v • 06:52 min • April 30th, 2019
April 30th, 2019 •
*1, *2, 2, 2, 2, 3, 3, 1, 2, 2
1Servizio di Allevamento e Sperimentazione Animale, Polo ScientificoTecnologico, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS)-Istituto Nazionale Ricovero e Cura Anziani (INRCA), 2Dipartimento di Scienze Cliniche e Molecolari, Università Politecnica delle Marche, 3UOS Centro di Terapia Cellulare "G. Lanzani", Azienda Socio Sanitaria Territoriale (ASST) Papa Giovanni XXIII
* These authors contributed equally

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Här presenterar vi en effektiv metod för att undersöka den antifibrotiska aktiviteten av intravenöst infunderade humana mesenkymala stromaceller som erhållits från hela navelsträngen efter induktion av lungskada av en endotrakeal injektion av bleomycin i C57BL /6 möss. Detta protokoll kan lätt utvidgas till prekliniska tester av andra Therapeutics.

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Immunologi och infektion bleomycin endotrakeal injektion lungskada mänskliga navelsträngen mesenkymala stromaceller (hUC-MSC) svans venen infusion C57BL/6 möss
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