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JoVE Journal Immunology and Infection
Inductie van muis long letsel door Endotracheale injectie van bleomycine
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Inductie van muis long letsel door Endotracheale injectie van bleomycine

Article DOI: 10.3791/58922-v • 06:52 min • April 30th, 2019
April 30th, 2019 •
*1, *2, 2, 2, 2, 3, 3, 1, 2, 2
1Servizio di Allevamento e Sperimentazione Animale, Polo ScientificoTecnologico, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS)-Istituto Nazionale Ricovero e Cura Anziani (INRCA), 2Dipartimento di Scienze Cliniche e Molecolari, Università Politecnica delle Marche, 3UOS Centro di Terapia Cellulare "G. Lanzani", Azienda Socio Sanitaria Territoriale (ASST) Papa Giovanni XXIII
* These authors contributed equally

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Hier presenteren we een effectieve methode voor het onderzoeken van de antifibrotische activiteit van intraveneus geïnfundeerde humane mesenchymale stromale cellen verkregen uit de gehele navelstreng na de inductie van long letsel door een Endotracheale injectie van bleomycine in C57BL /6 muizen. Dit protocol kan eenvoudig worden uitgebreid tot de preklinische testen van andere therapieën.

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Immunologie en infectie probleem 146 bleomycine Endotracheale injectie long letsel menselijke navelstreng mesenchymale stromale cellen (hUC-MSC) staart ader infusie C57BL/6 muizen
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