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Bewertung von Biomarkern in Gliom von Immunohistochemistry auf Paraffin-eingebetteten 3D Gliom Neurosphäre Kulturen
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Bewertung von Biomarkern in Gliom von Immunohistochemistry auf Paraffin-eingebetteten 3D Gliom Neurosphäre Kulturen

Article DOI: 10.3791/58931-v • 06:32 min • January 9th, 2019
January 9th, 2019 •
1,2, 2, 1,2, 1,3, 1,2, 1,2, 1,2
1Department of Neurosurgery, University of Michigan Medical School, 2Department of Cell & Developmental Biology, University of Michigan, 3INIBIOLP, Histology B-Pathology B, School of Medicine, UNLP

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Neurosphären als 3D Kulturen angebaut sind ein mächtiges Werkzeug um Gliom Biologie zu studieren. Hier präsentieren wir ein Protokoll zum Immunohistochemistry durchführen unter Beibehaltung der 3D-Struktur von Gliom Neurospheres durch Paraffin einbetten. Diese Methode ermöglicht die Charakterisierung von Gliom Neurosphäre Eigenschaften wie Stemness und neuronale Differenzierung.

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Cancer Research Ausgabe 143 gentechnisch verändert Gliom Biomarker Neurosphären 3D-Kulturen Immunostaining Paraffin einbetten
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