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JoVE Journal Cancer Research
Utvärdering av biomarkörer i gliom av immunohistokemi på Paraffin-inbäddat 3D gliom Neurosphere kulturer
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Utvärdering av biomarkörer i gliom av immunohistokemi på Paraffin-inbäddat 3D gliom Neurosphere kulturer

Article DOI: 10.3791/58931-v • 06:32 min • January 9th, 2019
January 9th, 2019 •
1,2, 2, 1,2, 1,3, 1,2, 1,2, 1,2
1Department of Neurosurgery, University of Michigan Medical School, 2Department of Cell & Developmental Biology, University of Michigan, 3INIBIOLP, Histology B-Pathology B, School of Medicine, UNLP

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Neurospheres odlas som 3D kulturer utgör ett kraftfullt verktyg för att studera gliom biologi. Här presenterar vi ett protokoll för att utföra immunohistokemi bibehållen 3D-strukturen för gliom neurospheres genom paraffin inbäddning. Denna metod gör det möjligt för karakterisering av gliom neurosphere egenskaper såsom stemness och neurala differentiering.

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Cancerforskning fråga 143 genetiskt modifierade gliom neurospheres 3D-kulturer immunfärgning biomarkörer paraffin inbäddning
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