Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / 非人类灵长类动物造血干细胞和祖细胞的制备及基因修饰…
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.

JoVE Journal Genetics
非人类灵长类动物造血干细胞和祖细胞的制备及基因修饰
Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Journal (Genetics)
Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification
Journal (Genetics)
High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification
 
Click here for the English version

非人类灵长类动物造血干细胞和祖细胞的制备及基因修饰

Article DOI: 10.3791/58933-v • 11:16 min • February 15th, 2019
February 15th, 2019 •
1, 1, 1, 1, 1, 1, 1,2,3, 1,2
1Stem Cell and Gene Therapy Program, Fred Hutchinson Cancer Research Center, 2Department of Medicine, University of Washington, 3Department of Pathology, University of Washington

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

该方案的目标是从启动的骨髓中分离出非人类灵长类动物 cd34+细胞, 用慢病毒载体对这些细胞进行基因修饰, 并准备一种产品, 用于注入自体宿主。协议的总长度约为48小时。

Tags

遗传学 第144期 造血干细胞和祖细胞 基因治疗 造血 非人类灵长类模型 细胞分离 慢病毒载体
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter