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Erkennung von Protease-Aktivität durch fluoreszierende Peptid Zymography
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Erkennung von Protease-Aktivität durch fluoreszierende Peptid Zymography

Article DOI: 10.3791/58938-v • 09:56 min • January 20th, 2019
January 20th, 2019 •
1,2, 1, 1,2
1Comprehensive Cancer Center, James Cancer Hospital and Solove Research Center, Ohio State University, 2Department of Biomedical Engineering, College of Engineering, Ohio State University

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Hier präsentieren wir Ihnen ein detailliertes Protokoll für eine modifizierte Zymographic-Technik in der fluoreszierenden Peptide als abbaubare Substrat anstelle von native Proteine verwendet werden. Elektrophorese von biologischen Proben in fluoreszierenden Peptid-Zymogramme ermöglicht die Detektion eines breiteren Spektrums von Proteasen als frühere Zymographic Techniken.

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Krebsforschung Ausgabe 143 Zymography fluoreszierende Peptid Protease Matrix-Metalloproteinase (MMP) kovalente Vernetzung Krebs konditioniert Medien Elektrophorese
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