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Påvisande av proteas aktivitet av fluorescerande peptid Zymography
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Påvisande av proteas aktivitet av fluorescerande peptid Zymography

Article DOI: 10.3791/58938-v • 09:56 min • January 20th, 2019
January 20th, 2019 •
1,2, 1, 1,2
1Comprehensive Cancer Center, James Cancer Hospital and Solove Research Center, Ohio State University, 2Department of Biomedical Engineering, College of Engineering, Ohio State University

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Här presenterar vi ett detaljerat protokoll för en modifierad zymographic teknik som används fluorescerande peptider som nedbrytbart substrat i stället för infödda proteiner. Elektrofores av biologiska prover i fluorescerande peptid zymograms möjliggör detektering av ett bredare utbud av proteaser än tidigare zymographic tekniker.

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Cancerforskning fråga 143 Zymography fluorescerande peptid proteashämmare Matrix metalloproteinas (MMP) kovalent Crosslinking Cancer luftkonditionerade Media elektrofores
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