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Påvisning af Protease aktivitet af fluorescerende peptid Zymography
 
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Påvisning af Protease aktivitet af fluorescerende peptid Zymography

Article doi: 10.3791/58938
January 20th, 2019

Summary January 20th, 2019

Please note that all translations are automatically generated.

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Vi præsenterer her, en detaljeret protokol for en modificeret zymographic teknik hvor fluorescerende peptider bruges som de nedbrydelige substrat i stedet for native proteiner. Elektroforese af biologiske prøver i fluorescerende peptid zymograms giver mulighed for påvisning af en bredere vifte af proteaser end tidligere zymographic teknikker.

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