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Påvisning af Protease aktivitet af fluorescerende peptid Zymography
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Påvisning af Protease aktivitet af fluorescerende peptid Zymography

Article DOI: 10.3791/58938-v • 09:56 min • January 20th, 2019
January 20th, 2019 •
1,2, 1, 1,2
1Comprehensive Cancer Center, James Cancer Hospital and Solove Research Center, Ohio State University, 2Department of Biomedical Engineering, College of Engineering, Ohio State University

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Vi præsenterer her, en detaljeret protokol for en modificeret zymographic teknik hvor fluorescerende peptider bruges som de nedbrydelige substrat i stedet for native proteiner. Elektroforese af biologiske prøver i fluorescerende peptid zymograms giver mulighed for påvisning af en bredere vifte af proteaser end tidligere zymographic teknikker.

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Kræftforskning spørgsmålet 143 Zymography fluorescerende peptid Protease Matrix Metalloproteinase (MMP) kovalent Crosslinking kræft aircondition medier elektroforese
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