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Opsporen van Protease-activiteit door fluorescerende Peptide Zymography

Article DOI: 10.3791/58938-v • 09:56 min • January 20th, 2019
January 20th, 2019 •
1,2, 1, 1,2
1Comprehensive Cancer Center, James Cancer Hospital and Solove Research Center, Ohio State University, 2Department of Biomedical Engineering, College of Engineering, Ohio State University

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Hier presenteren we een gedetailleerd protocol voor een gewijzigde zymographic techniek waarin fluorescerende peptides worden gebruikt als het afbreekbaar substraat in plaats van de inheemse proteïnen. Elektroforese van biologische monsters in fluorescerende peptide zymograms maakt detectie van een breder scala van proteasen dan vorige zymographic technieken.

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Kankeronderzoek kwestie 143 Zymography fluorescerende Peptide Protease Matrix Metalloproteinase (MMP) covalente Crosslinking kanker geconditioneerd Media elektroforese
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