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JoVE Journal Neuroscience
Identifisere Cell Surface markører for primær neural Stem og stamceller ved metabolsk merking av Sialoglycan
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Identifisere Cell Surface markører for primær neural Stem og stamceller ved metabolsk merking av Sialoglycan

Article DOI: 10.3791/58945-v • 11:39 min • September 7th, 2019
September 7th, 2019 •
*1, *2, 1
1Brain and Spinal Cord Innovative Research Center of Tongji Hospital, School of Life Sciences and Technology, Tongji University and Frontier Science Research Center for Stem Cells of Ministry of Education, 2College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, and Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University
* These authors contributed equally

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Presentert her er en protokoll som kombinerer en in vitro neural-endothelial co-kultur system og metabolsk innlemmelse av sialoglycan med bioorthogonal funksjonelle grupper for å utvide primære nevrale stammen og stamceller og merke deres overflate sialoglycoproteins for bildebehandling eller masse-massespektrometri analyse av celleoverflaten markører.

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Nevrovitenskap metabolsk glycan merking Sialic syrer bioorthogonal reaksjon nevrale stamceller stamceller overflate markører endothelial celler proteom kjemisk merking cerebral cortex
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