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Journal / Bioengineering / 使用自动微生物反应器系统从中国仓鼠卵巢细胞中纯化和分析单克隆抗体…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
使用自动微生物反应器系统从中国仓鼠卵巢细胞中纯化和分析单克隆抗体
Construction and Setup of a Bench-scale Algal Photosynthetic Bioreactor with Temperature, Light, and pH Monitoring for Kinetic Growth Tests
Journal (Bioengineering)
Construction and Setup of a Bench-scale Algal Photosynthetic Bioreactor with Temperature, Light, and pH Monitoring for Kinetic Growth Tests
Double Emulsion Generation Using a Polydimethylsiloxane (PDMS) Co-axial Flow Focus Device
Journal (Bioengineering)
Double Emulsion Generation Using a Polydimethylsiloxane (PDMS) Co-axial Flow Focus Device
 
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使用自动微生物反应器系统从中国仓鼠卵巢细胞中纯化和分析单克隆抗体

Article DOI: 10.3791/58947-v • 10:50 min • May 1st, 2019
May 1st, 2019 •
1, 1, 1, 1, 1, 1, 1, 1
1Center for Drug Evaluation and Research, Office of Product Quality, Office of Biotechnology Products, Division of Biotechnology Review and Research II, U.S. Food and Drug Administration

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介绍了从自动微生物反应器的收获细胞培养液(HCCF)中分离和后续分析的单克隆抗体的详细方案。还介绍了使用分析来确定关键质量属性 (CQA) 和最大化有限样本量以提取重要信息。

Tags

生物工程,第147期,微生物反应器,中国仓鼠卵巢细胞,细胞培养,筛选,甘油,单克隆抗体,大小排除色谱,多角度光散射
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