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Journal / Immunology and Infection / Merkel celle Polyomavirus infektion og påvisning
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Immunology and Infection

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JoVE Journal Immunology and Infection
Merkel celle Polyomavirus infektion og påvisning
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Merkel celle Polyomavirus infektion og påvisning

Article DOI: 10.3791/58950-v • 13:45 min • February 7th, 2019
February 7th, 2019 •
*1, *1, 2, 1
1Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, 2Laboratory of Cellular Oncology, National Cancer Institute
* These authors contributed equally

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Vi præsenterer her, en protokol for at inficere primære human dermal fibroblast med MCPyV. Protokollen omfatter isolering af dermal fibroblaster, udarbejdelse af MCPyV virioner, virusinfektion, immunofluorescens farvning og fluorescens i situ hybridisering. Denne protokol kan udvides til kendetegner MCPyV-værtssammenspil og opdage andre inficerbare celletyper ved MCPyV.

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Immunologi og infektion spørgsmålet 144 Merkel celler polyomavirus isolation dermal fibroblaster infektion collagenase IV CHIR99021 immunfluorescent farvning fluorescens i situ hybridisering in situ DNA-hybridisering kædereaktion transskription replikering
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