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JoVE Journal Biochemistry
Analisi della piegatura della proteina, trasporto e degradazione in cellule viventi di radioattivo Pulse Chase
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Analisi della piegatura della proteina, trasporto e degradazione in cellule viventi di radioattivo Pulse Chase

Article DOI: 10.3791/58952-v • 08:59 min • February 12th, 2019
February 12th, 2019 •
1,2, 1, 1, 1, 1, 1
1Cellular Protein Chemistry, Utrecht University, 2Program in Cellular and Molecular Medicine, Boston Children's Hospital

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Qui descriviamo un protocollo per un metodo generale di pulse-chase che permette l'analisi cinetica di pieghevole, trasporto e degradazione delle proteine da seguire in cellule vive.

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Proteina di biochimica problema 144 pieghevole radiolabeling SDS-PAGE immunoprecipitazione impulso inseguire analisi conformazionale cinetica
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