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JoVE Journal Neuroscience
Un protocolo de acero inoxidable para el aislamiento de ARN de alta calidad del Laser capturar las células Microdissected de Purkinje en el cerebelo humano Post-Mortem
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Un protocolo de acero inoxidable para el aislamiento de ARN de alta calidad del Laser capturar las células Microdissected de Purkinje en el cerebelo humano Post-Mortem

Article DOI: 10.3791/58953-v • 09:04 min • January 17th, 2019
January 17th, 2019 •
1, 2,3,4, 1
1Department of Pathology and Cell Biology, Columbia University, 2Division of Movement Disorders, Department of Neurology, Yale University, 3Department of Chronic Disease Epidemiology, Yale School of Public Health, Yale University, 4Center for Neuroepidemiology and Clinical Neurological Research, Yale School of Medicine, Yale University

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Este protocolo utiliza un enfoque libre de manchas para visualizar y aislar a las células de Purkinje en tejido fresco congelado del cerebelo humano post-mortem mediante microdisección de captura láser. El propósito de este protocolo es generar cantidades suficientes de ARN de alta calidad para secuenciación del RNA.

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Neurociencia número 143 células de Purkinje de cerebelo laser microdissection de la captura RNA RNAseq temblor esencial
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