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JoVE Journal
Bioengineering

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用于全基因组 DNA 分析的超长读数测序
 
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用于全基因组 DNA 分析的超长读数测序

Article doi: 10.3791/58954
March 15th, 2019

Summary March 15th, 2019

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长读序列极大地促进了复杂基因组的组合和结构变异的表征。我们描述了一种利用纳米粒子测序平台生成超长序列的方法。该方法采用优化的 DNA 提取, 然后进行改进的文库准备, 从人体细胞中产生数百条覆盖适度的千列酶读数。

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