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Journal / Immunology and Infection / Diskriminering av syv immun celle undergrupper av …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Diskriminering av syv immun celle undergrupper av to-fluorochrome Flow cytometri
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Journal (Immunology and Infection)
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Diskriminering av syv immun celle undergrupper av to-fluorochrome Flow cytometri

Article DOI: 10.3791/58955-v • 10:58 min • March 5th, 2019
March 5th, 2019 •
1, 2
1Oncology Research, Medimmune, LLC, 2Division of Rheumatology, Johns Hopkins University School of Medicine

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Her presenterer vi en flyt cytometric protokoll for å identifisere CD4+ og CD8+ T celler, γδ T celler, B celler, NK celler og monocytter i perifert menneskeblod med bare to fluorochromes i stedet for syv. Med denne tilnærmingen, kan fem ekstra markører registreres på de fleste flyt cytometers.

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Immunologi og infeksjon problemet 145 flowcytometri immunologi immunophenotyping menneskelige perifert blod fluorochrome tålmodig multiparametric analyse.
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