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Journal / Biochemistry / Human perifert blod Neutrofile Isolation for at…
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Biochemistry
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JoVE Journal Biochemistry
Human perifert blod Neutrofile Isolation for at forhøre Parkinsons Associated LRRK2 Kinase Pathway ved at vurdere Rab10 fosforylering
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Human perifert blod Neutrofile Isolation for at forhøre Parkinsons Associated LRRK2 Kinase Pathway ved at vurdere Rab10 fosforylering

Article DOI: 10.3791/58956-v • 12:49 min • March 21st, 2020
March 21st, 2020 •
*1, *1, 2, 2, 2, 3, 4, 5, 1, 1,6
1MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, 2The Michael J. Fox Foundation for Parkinson's Research, 3Department of Medical and Molecular Genetics, Indiana University School of Medicine, 4Morton and Gloria Shulman Movement Disorders Centre and the Edmond J. Safra Program in Parkinson's Disease, Toronto Western Hospital, University of Toronto, 5Division of Cell Signalling and Immunology, School of Life Sciences, University of Dundee, 6Division of Molecular and Clinical Medicine, School of Medicine, Ninewells Hospital and Medical School, University of Dundee
* These authors contributed equally

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Mutationer i leucin rige gentage kinase 2 gen (LRRK2) forårsage arvelig Parkinsons sygdom. Vi har udviklet en nem og robust metode til vurdering af LRRK2-kontrolleret fosforylering af Rab10 hos menneskers perifere blodneutrofiler. Dette kan hjælpe med at identificere personer med øget LRRK2 kinase pathway aktivitet.

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Biokemi Problem 157 Parkinsons sygdom biomarkører LRRK2 kinase perifere blodneutrofiler Rab-proteiner kalvelagshandel proteinfosforylering
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