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Journal / Neuroscience / Produktion, oprensning og kvalitetskontrol for…
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Neuroscience

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JoVE Journal Neuroscience
Produktion, oprensning og kvalitetskontrol for Adeno-associeret Virus-baseret vektorer
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Produktion, oprensning og kvalitetskontrol for Adeno-associeret Virus-baseret vektorer

Article DOI: 10.3791/58960-v • 09:21 min • January 29th, 2019
January 29th, 2019 •
*1,2, *1,2, *1,2, 1,2, 1,2,3
1VIB-KU Leuven Center for Brain and Disease Research, 2Department of Neuroscience, KU Leuven, 3Leuven Brain Institute
* These authors contributed equally

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Her, beskriver vi en strategi for effektiv og reproducerbar til at producere, titer og kvalitetskontrol partier af adeno-associeret virus vektorer. Det giver brugeren mulighed for opnå en vektor forberedelse med høj titer (≥ 1 x 1013 vektor genomer/mL) og en høj renhed, klar til in vitro- brug eller i vivo .

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Neurovidenskab spørgsmålet 143 Adeno-associeret virus (AAV)-baseret vektorer iodixanol gradient gen levering centralnervesystemet blod-hjerne-barrieren PHP. B kapsid
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