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Journal / Neuroscience / Produksjon, rensing og kvalitetskontroll for…
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Neuroscience

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JoVE Journal Neuroscience
Produksjon, rensing og kvalitetskontroll for Adeno-assosiert Virus-basert vektorer
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Produksjon, rensing og kvalitetskontroll for Adeno-assosiert Virus-basert vektorer

Article DOI: 10.3791/58960-v • 09:21 min • January 29th, 2019
January 29th, 2019 •
*1,2, *1,2, *1,2, 1,2, 1,2,3
1VIB-KU Leuven Center for Brain and Disease Research, 2Department of Neuroscience, KU Leuven, 3Leuven Brain Institute
* These authors contributed equally

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Her beskriver vi en effektiv og reproduserbar strategi å produsere titer og kvalitetskontroll grupper av adeno-assosiert virus vektorer. Det lar brukeren få en vektor forberedelse med høy-titer (≥1 x 1013 vektor genomer/mL) og en høy renhet, klar til bruk i vitro eller vivo .

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Nevrovitenskap problemet 143 Adeno-assosiert virus (AAV)-basert vektorer iodixanol gradering gen levering sentralnervesystemet blod-hjerne-barrieren PHP. B kapsid
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