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Primær cellekultur af renset GABAergic eller Glutamatergic neuroner etableret gennem fluorescens-aktiveret celle sortering

Article DOI: 10.3791/58974-v • 10:57 min • June 6th, 2019
June 6th, 2019 •
1, 1, 2, 3, 1
1Institut für Integrative Neuroanatomie, Charité, Universitätsmedizin Berlin, 2Department for Cytometry and Cellsorting, German Rheumatism Research Center, Leibniz Institute, 3Departments of Genetic and Behavioral Neuroscience, Graduate School of Medicine, Gunma University

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Denne protokol beskriver en celle sortering baseret metode til rensning og kultur af fluorescerende GABAergic eller glutamatergic neuroner fra neocortex og hippocampus af postnatale mus eller rotter.

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Neurovidenskab FACS cellekultur GABAergic neuroner interneuroner glutamatergic neuroner vigtigste celler primære neuroner hippocampus cortex mus rotte
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