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Neuroscience

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JoVE Journal Neuroscience
Cultura de células primárias de neurônios Gabaérgicos ou glutamatérgicos purificados estabelecidos através da triagem de células ativadas por fluorescência
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Cultura de células primárias de neurônios Gabaérgicos ou glutamatérgicos purificados estabelecidos através da triagem de células ativadas por fluorescência

Article DOI: 10.3791/58974-v • 10:57 min • June 6th, 2019
June 6th, 2019 •
1, 1, 2, 3, 1
1Institut für Integrative Neuroanatomie, Charité, Universitätsmedizin Berlin, 2Department for Cytometry and Cellsorting, German Rheumatism Research Center, Leibniz Institute, 3Departments of Genetic and Behavioral Neuroscience, Graduate School of Medicine, Gunma University

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Este protocolo descreve um método baseado classificação da pilha para a purificação e a cultura de neurônios gabaergic ou glutamatérgico fluorescentes do neocórtex e do hipocampo de ratos ou de ratos pós-natal.

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Neurociência edição 148 FACS cultura celular neurônios Gabaérgicos interneurônios neurônios glutamatérgicos células principais neurônios primários hipocampo córtex rato rato
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