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JoVE Journal Developmental Biology
Effektiv differentiering af humane pluripotente stamceller i leverceller
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Effektiv differentiering af humane pluripotente stamceller i leverceller

Article DOI: 10.3791/58975-v • 07:37 min • June 11th, 2019
June 11th, 2019 •
1,2, 1, 1
1Institute for Stem Cell Biology & Regenerative Medicine, Stanford-UC Berkeley Siebel Stem Cell Institute, Stanford University School of Medicine, 2Department of Developmental Biology, Stanford University School of Medicine

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Denne protokol beskriver en monolayer, serum-fri metode til effektivt at generere hepatocyte-lignende celler fra humane pluripotente stamceller (hPSCs) i 18 dage. Dette indebærer seks trin som hpscs sekventielt differentiere til mellemliggende celle-typer såsom primitive streak, definitive endoderm, posterior foregut og lever opløbet forfædre før dannelse hepatocyte-lignende celler.

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Udviklingsmæssige biologi human pluripotente stamcelle effektiv differentiering endoderm lever stamfader hepatocyt
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