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Journal / Developmental Biology / ヒト多能性幹細胞の肝細胞への効率的な分化…
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Developmental Biology

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JoVE Journal Developmental Biology
ヒト多能性幹細胞の肝細胞への効率的な分化
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ヒト多能性幹細胞の肝細胞への効率的な分化

Article DOI: 10.3791/58975-v • 07:37 min • June 11th, 2019
June 11th, 2019 •
1,2, 1, 1
1Institute for Stem Cell Biology & Regenerative Medicine, Stanford-UC Berkeley Siebel Stem Cell Institute, Stanford University School of Medicine, 2Department of Developmental Biology, Stanford University School of Medicine

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このプロトコルは、ヒト多能性幹細胞(hPSC)から肝細胞様細胞を18日間で効率的に生成する単層、血清フリー法を詳細に述べている。これは、hPSCが肝細胞様細胞を形成する前に、原始的な筋、決定的な内因性内因性、後部前腸および肝芽前駆体のような中間細胞型に順次分化するように6つのステップを伴う。

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発生生物学 問題 148 ヒト 多能性幹細胞 効率的 分化 内皮 肝臓 前駆体 肝細胞
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