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Journal / Genetics / Genetische manipulatie van Dictyostelium…
JoVE Journal
Genetics

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JoVE Journal Genetics
Genetische manipulatie van Dictyostelium discoideum cellen op basis van de selectie en de groei van bacteriën
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Genetische manipulatie van Dictyostelium discoideum cellen op basis van de selectie en de groei van bacteriën

Article DOI: 10.3791/58981-v • 06:08 min • January 25th, 2019
January 25th, 2019 •
1, 2, 1, 3, 3, 4,5, 1, 1
1MRC Laboratory of Molecular Biology, 2Department of Molecular and Cell Biology, University of Connecticut, 3Cancer Research UK Beatson Institute Glasgow, 4MRC Laboratory for Molecular Cell Biology, University College London, 5Department of Cell and Developmental Biology, University College London

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Dictyostelium-discoideum is een populair model-organisme te bestuderen van complexe cellulaire processen zoals cel migratie, endocytose en ontwikkeling. Het nut van het organisme is afhankelijk van de haalbaarheid van genetische manipulatie. Hier presenteren we methoden om te transfect Dictyostelium discoideum cellen die bestaande van kweken cellen in vloeibare media beperkingen.

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Genetica kwestie 143 Dictyostelium transfectie knock-out knock-in extrachromosomal plasmiden act5 overexpressie chemotaxis beweeglijkheid macropinocytosis
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