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JoVE Journal
Immunology and Infection

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在生物安全2级环境中制备假型颗粒研究高致病性冠状病毒
 
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在生物安全2级环境中制备假型颗粒研究高致病性冠状病毒

Article DOI: 10.3791/59010-v
March 1st, 2019

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Summary March 1st, 2019

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Click here for the English version.

在这里, 我们提出了一个协议, 以产生伪型粒子在 bsl-2 设置纳入高致病性病毒中东呼吸综合征和严重急性呼吸综合征冠状病毒的尖峰蛋白。这些伪类型粒子包含一个荧光素酶报告基因, 允许病毒进入目标宿主细胞的定量。

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