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JoVE Journal
Immunology and Infection

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Produktion af Pseudotyped partikler at studere højpatogen coronavirus i biosikkerhed niveau 2 omgivelser
 
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Produktion af Pseudotyped partikler at studere højpatogen coronavirus i biosikkerhed niveau 2 omgivelser

Article DOI: 10.3791/59010-v
March 1st, 2019

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Summary March 1st, 2019

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Vi præsenterer her, en protokol til at generere pseudotyped partikler i BSL-2 omgivelser indarbejde spike protein af højpatogene virus Mellemøsten respiratorisk syndrom og svær Akut respiratorisk syndrom coronavirus. Disse pseudotyped partikler indeholder et luciferase reporter gen giver mulighed for kvantificering af virus ibrugtagning vært målceller.

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