Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Produzione di particelle pseudotipizzate per…
JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection
Produzione di particelle pseudotipizzate per studiare la patogenicità coronavirus in un ambiente di livello 2 di biosicurezza
Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Journal (Immunology and Infection)
Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Longitudinal Follow-Up of Urinary Tract Infections and Their Treatment in Mice using Bioluminescence Imaging
Journal (Immunology and Infection)
Longitudinal Follow-Up of Urinary Tract Infections and Their Treatment in Mice using Bioluminescence Imaging
 
Click here for the English version

Produzione di particelle pseudotipizzate per studiare la patogenicità coronavirus in un ambiente di livello 2 di biosicurezza

Article DOI: 10.3791/59010-v • 08:40 min • March 1st, 2019
March 1st, 2019 •
1,2, 3, 3, 4, 3,5, 3, 1
1Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, 2INRA, Virologie et Immunologie Moléculaires, 3Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, 4Department of Microbiology, College of Agricultural and Life Sciences, Cornell University, 5Horae Gene Therapy Center, University of Massachusetts Medical School

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Qui, presentiamo un protocollo per generare particelle pseudotipizzate in un ambiente di BSL-2 che incorporano la proteina spike della sindrome respiratoria Medio Oriente di virus ad alta patogenicità e coronavirus della sindrome respiratoria acuta grave. Queste particelle pseudotipizzate contengono un gene del reporter di luciferase che permette la quantificazione dell'entrata del virus in cellule bersaglio.

Tags

Ricerca sul cancro problema 145 particella Pseudotipizzata pseudovirion coronavirus CoV spike proteine virus della leucemia murina sindrome respiratoria acuta grave coronavirus SARS-CoV Medio Oriente sindrome respiratoria coronavirus MERS-CoV MLV
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter