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Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions

Article DOI: 10.3791/59016-v • 04:33 min • March 12th, 2019
March 12th, 2019 •
1,2, 3
1Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, 2Department of Anatomy, School of Medicine, Kaohsiung Medical University, 3Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University

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The hormone secreted by the adrenal cortex is vital for animals against stress and diseases. Here, we present a protocol to culture the primary rat adrenal cells. It could be a good in vitro platform for investigating the mechanisms of the reagent of interest in adrenal steroidogenesis and lipid biosynthesis.

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Primary Culture Rat Adrenocortical Cells Steroidogenic Functions Adrenal Steroidogenesis Stress-related Diseases Inflammatory Responses Wei-Chang Lee Sterilizing SD Rat Plastic Plate Supine Position External Curved Forceps Blunt Dissection Y Shape Muscle Cutting Left Adrenal Gland Right Adrenal Gland Stomach And Liver Isolation Sterile Dish Adrenal Capsule Removal Small Tissue Pieces Serum-free DMEM F-12 Medium
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