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JoVE Journal Genetics
Ved hjælp af in Vitro og i celler form at undersøge lille molekyle induceret Pre-mRNA strukturelle ændringer
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Ved hjælp af in Vitro og i celler form at undersøge lille molekyle induceret Pre-mRNA strukturelle ændringer

Article DOI: 10.3791/59021-v • 11:58 min • January 30th, 2019
January 30th, 2019 •
1, 2, 1
1Calibr, The Scripps Research Institute, 2Department of Integrative Structural and Computational Biology, The Scripps Research Institute

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Detaljerede protokoller for både in vitro og i celle selektiv 2'-hydroxyl acylation analyseret af primer forlængelse (SHAPE) forsøg at bestemme sekundær struktur af pre-mRNA sekvenser af interesse i overværelse af et RNA-targeting lille molekyle er præsenteret i denne artikel.

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Denne måned i JoVE spørgsmålet 143 lille molekyle RNA-targeting RNA struktur in vitro- figur figur spinal muskelatrofi RG-7916 i celle
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