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Journal / Genetics / Bruke i Vitro og i celle form å undersøke små…
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Genetics

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JoVE Journal Genetics
Bruke i Vitro og i celle form å undersøke små molekyl indusert Pre-mRNA strukturelle endringer
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Bruke i Vitro og i celle form å undersøke små molekyl indusert Pre-mRNA strukturelle endringer

Article DOI: 10.3791/59021-v • 11:58 min • January 30th, 2019
January 30th, 2019 •
1, 2, 1
1Calibr, The Scripps Research Institute, 2Department of Integrative Structural and Computational Biology, The Scripps Research Institute

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Detaljert protokoller for både in vitro og i celle selektiv 2-hydroksyl acylation analysert av primer forlengelsen (SHAPE) eksperimenter for å finne den sekundære strukturen i pre-mRNA sekvenser av interesse i nærvær av et RNA målretting små molekyl er presentert i denne artikkelen.

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Denne måneden i JoVE problemet 143 små molekyl RNA målretting RNA struktur i vitro form form spinal muskel atrofi RG-7916 i celler
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