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JoVE Journal Genetics
Met behulp van In Vitro en In cel vorm te onderzoeken klein molecuul veroorzaakte structurele veranderingen van de Pre-mRNA
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Met behulp van In Vitro en In cel vorm te onderzoeken klein molecuul veroorzaakte structurele veranderingen van de Pre-mRNA

Article DOI: 10.3791/59021-v • 11:58 min • January 30th, 2019
January 30th, 2019 •
1, 2, 1
1Calibr, The Scripps Research Institute, 2Department of Integrative Structural and Computational Biology, The Scripps Research Institute

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Er zijn gedetailleerde protocollen voor beide in vitro en in cel selectieve 2'-hydroxyl acylering geanalyseerd door primer extensie (SHAPE) experimenten om de secundaire structuur van pre-mRNA opeenvolgingen van belang in de aanwezigheid van een kleine molecule van RNA-targeting in dit artikel wordt gepresenteerd.

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Deze maand in JoVE kwestie 143 klein molecuul RNA-targeting RNA structuur in vitro vorm vorm spinale musculaire atrofie RG-7916 in cel
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