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Journal / Biochemistry / Celle fraksjoneres av U937 celler i fravær av…
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Biochemistry

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JoVE Journal Biochemistry
Celle fraksjoneres av U937 celler i fravær av høyhastighets sentrifugering
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Journal (Biochemistry)
Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin
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Journal (Biochemistry)
Arteriovenous Blood Sampling Technique in Mice Using Sulzer's Vein and Left Ventricle for Metabolomic Analysis (Video) | JoVE
 
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Celle fraksjoneres av U937 celler i fravær av høyhastighets sentrifugering

Article DOI: 10.3791/59022-v • 06:53 min • January 18th, 2019
January 18th, 2019 •
1, 1, 1, 1, 1, 1
1Department of Basic and Clinical Sciences, Albany College of Pharmacy and Health Sciences

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Her presenterer vi en protokoll for å isolere plasma membran, cytoplasma og mitokondrier U937 celler uten bruk av høyhastighets sentrifugering. Denne teknikken kan brukes til å rense subcellular brøker for påfølgende undersøkelse av protein lokalisering via immunoblotting.

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Biokjemi problemet 143 Cellular fraksjoneres mitokondrier membran cytoplasma Organelles sentrifugering U937
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