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Journal / Bioengineering / Håndtering og vurdering af menneskers primære…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Håndtering og vurdering af menneskers primære prostata Organoid kultur
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Håndtering og vurdering af menneskers primære prostata Organoid kultur

Article DOI: 10.3791/59051-v • 08:45 min • January 17th, 2019
January 17th, 2019 •
*1, *1, 1, 1,2,3, 1,3
1Department of Pathology, University of Illinois at Chicago, 2Departments of Urology, Physiology, and Biophysics, University of Illinois at Chicago, 3University of Illinois Cancer Center
* These authors contributed equally

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Vi præsenterer her, en protokol for at lede menneskers primære prostata organoid håndtering så foreslår slutpunkter for at vurdere fænotype. Såning, kultur vedligeholdelse, recovery fra matrix gel, er morfologiske kvantificering, integrering og skæring, FFPE skæring, hele-mount farvning og anvendelse af kommercielle assays beskrevet.

Tags

Bioteknologi spørgsmålet 143 Organoid slutpunkt 3D quanification billedanalyse FFPE hele-mount morfologi prostata menneskelige immunofluorescens
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