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Síntese e Bioconjugation dos reagentes Thiol-reativa para a criação de Site-seletivamente modificado Immunoconjugates
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Síntese e Bioconjugation dos reagentes Thiol-reativa para a criação de Site-seletivamente modificado Immunoconjugates

Article DOI: 10.3791/59063-v • 08:47 min • March 6th, 2019
March 6th, 2019 •
1, 1,2, 1, 1,2,3,4
1Department of Chemistry, Hunter College of the City University of New York, 2Ph.D. Program in Chemistry, Graduate Center of the City University of New York, 3Department of Radiology, Memorial Sloan Kettering Cancer Center, 4Department of Radiology, Weill Cornell Medical College

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Neste protocolo, descreveremos a síntese de vagens, phenyoxadiazolyl metil sulfona-baseado reagente para a ligação local-seletivo de cargas para os tióis de biomoléculas, especialmente de anticorpos. Além disso, descreveremos a síntese e caracterização de um quelante bifuncional PODS-rolamento e sua conjugação com um anticorpo de modelo.

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Química edição 145 bioconjugation site-specific local-seletivo bioconjugation maleimide tiol sulfidrila radioimmunoconjugate immunoconjugate
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