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JoVE Journal Cancer Research
Gær som et chassis til udvikling af funktionelle assays til undersøgelse af humane P53
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Gær som et chassis til udvikling af funktionelle assays til undersøgelse af humane P53

Article DOI: 10.3791/59071-v • 14:57 min • August 4th, 2019
August 4th, 2019 •
1, 2, 3, *3, *1, *2
1Mutagenesis and Cancer Prevention Unit, Ospedale Policlinico San Martino, 2Department CIBIO, University of Trento, 3LAQV/REQUIMTE, Laboratory of Microbiology, Faculty of Pharmacy, University of Porto
* These authors contributed equally

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Præsenteret her er fire protokoller til at konstruere og udnytte gær Saccharomyces cerevisiae reporter stammer til at studere Human P53 transaktiverende potentiale, virkningerne af sine forskellige Cancer-associerede mutationer, co-udtrykte interagerende proteiner, og virkningerne af specifikke små molekyler.

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Kræftforskning P53 gær respons element funktionel analyse transkriptionen væksthæmning
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