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Journal / Biochemistry / Real-time overvågning af DNA streng Exchange…
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Biochemistry

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JoVE Journal Biochemistry
Real-time overvågning af DNA streng Exchange reaktion medieret af Rad51
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Real-time overvågning af DNA streng Exchange reaktion medieret af Rad51

Article DOI: 10.3791/59073-v • 06:24 min • February 13th, 2019
February 13th, 2019 •
1, 1, 1, 1
1Cell Biology Center, Institute of Innovative Research, Tokyo Institute of Technology

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Fluorescens resonans energi transfer-baseret real-time observation systemer af DNA streng exchange reaktion ved Rad51 mellemkomst blev udviklet. Bruger de protokoller, der præsenteres her, er vi købedygtig opdager dannelsen af reaktion mellemprodukter og deres omdannelse til produkter, mens også analysere den enzymatiske kinetik af reaktionen.

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Biokemi spørgsmålet 144 homologe rekombination DNA streng exchange reaktion Rad51 Swi5-Sfr1 real-time observation fluorescens resonans energioverførsel (FRET)
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